a) You can save the annotation to the tiff file with AnnoSaveToTiff method from BiAnno.ocx. (There is code in the Annotation VB sample frmImage.vb file)
b) You can save the annotation to another file with AnnoSaveToFile method from BiAnno.ocx. This is useful for non TIFF images. For example you have a bmp image. You put some annotations to the bmp and then save annotations to xyz.ann file. You can load annotations from xyz.ann to the bmp with AnnoLoadFromFile method (in BiAnno.ocx). (There is a code in the Annotation VB sample mdiform1.vb file)

Regards,
Technical Support Team